The autolyse technique can bring many benefits to your bread dough. This post goes into how to autolyse, but also when not to. Various – Autolyse. Label: Show Me Your Wounds Production – LESION # Format: CD, Compilation, Limited Edition, Numbered. Country. In biology, autolysis, more commonly known as self-digestion, refers to the destruction of a Limited synthesis of adenosine triphosphate impairs many cellular. Hi everyone,I would like to know your experience with different autolysis durations and temperatures for sourdough.1) What is the longest. I don't know how many hours you can do an autolyse with no salt at room temperature before you run into these issues. Autolysis with fat. I have had several people ask me about this. So, here we are. Another experiment to see why we should or should not do it. All that autolyse means is to mix the water and flour - without anything else - and leave it for that 20 to 30 minutes or several hours - so. They all produced good breads, but since I've never done side by side tests, I have no idea if there were significant differences. At the moment. You might have seen the autolyse method mentioned in the steps of a recipe. to combine all the flour with water, as its important it all gets hydrated. There are two main enzymes and they both do different things: Protease enzymes start to break down proteins in the flour. This is what helps the dough become.